OUR TECHNICS

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NEURAL STEM CELLS

Adult neural stem cells
Primary neural stem cells

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AUTOPHAGY SIGNALING

Kinase signaling pathways
Subcellular organelles
Adaptor proteins

Scientist in the Lab

IMMUNE CELLS

Primary Microglia
Primary macrophages

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AMYLOID BETA DEGRADATION ANALYSIS

Microglia

Phagocytosis
Amyloid beta

Closeup of a Petri Dish

INFLAMMASOME ASSAY

IL-1beta, IL-18
Caspase-1
ASC

Scientist in the Lab

ELISA

Cytokines analysis
Phospholipid analysis
Amyloid beta analysis

Embryonic Stem Cells

MICE

SPF (specific-pathogen free) mice
semi-SPF mice

Embryonic Stem Cells

TRANSGENIC MICE

Cre/loxP system

Microscope

MICE BEHAVIOR TEST

Open field test

Morris water maze

Y-maze

Social interaction test

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PROTEIN EXPRESSION ANALYSIS

Western blotting

Embryonic Stem Cells

GENE EXPRESSION ANALYSIS

PCR

Closeup of a Petri Dish

CELL IMAGING

Immunocytochemistry

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TISSUE IMAGING

immunohistochemistry

Molecule

LIVE CELL IMAGING

Mitochondrial analysis
Calcium imaging

Microscope

GENOME ENGINNERING

CRIPSR/Cas9

Scientist in the Lab

CELLULAR GENE EXPRESSION

Gene overexpression
RNAi

Closeup of a Petri Dish

VIRUS PRODUCTION

AAV
Lenti virus

Petri Dish

CLONING

Competent cells
Restriction enzyme
Ligation
Site-directed mutagenesis

Molecule

AUTOPHAGY ASSAY

LC3 western blotting
LC3 puncta analysis

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CELL DEATH ASSAY

Hoechst / PI analysis

Embryonic Stem Cells

CELL SORTING ANALYSIS

FACS
Acute cell isolation

Molecule

EX VIVO ANALYSIS

Hippocampal analysis